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Overview
This protocol explains how to measure residual water-loss (gres) and its phase transition (Tp) using the DroughtBox located at the Hawkesbury Institute for the Environment. This box and the code was develop by Craig Barton.
Background
Units, Terms and Definitions
| Term
|
Definition
|
Units
|
| gmin
|
Leaf minimum conductance
|
mmol.m−2 s−1
|
| gbark
|
Residual water-loss from small branches.
|
mmol.m−2 s−1
|
| gres
|
Residual water-loss from small branches and leaves. Is the sum between gmin and gbark
|
mmol.m−2 s−1
|
| VPD
|
Vapor pressure deficit
|
kPa
|
| Tp
|
Phase transition temperature (Tp), beyond which gres significantly increases.
|
Degree Celsius
|
| w
|
Branch weight
|
grams
|
| BA
|
Surface area of the stem determined from measurements of stem length and basal diameter and assuming the shape of a cone.
|
m2
|
| LA
|
Leaf area
|
m2
|
| E
|
Transpiration
|
mmol.m−2 s−1
|
Links to resources and suppliers
Literature references
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Billon, L. M., Blackman, C. J., Cochard, H., Badel, E., Hitmi, A., Cartailler, J., ... & Torres‐Ruiz, J. M. (2020). The DroughtBox: a new tool for phenotyping residual branch conductance and its temperature dependence during drought (Vol. 43, No. 6, pp. 1584-1594). Chichester, UK: John Wiley & Sons, Ltd.
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Bueno, A., Alfarhan, A., Arand, K., Burghardt, M., Deininger, A. C., Hedrich, R., ... & Riederer, M. (2019). Effects of temperature on the cuticular transpiration barrier of two desert plants with water-spender and water-saver strategies. Journal of Experimental Botany, 70(5), 1613-1625.
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Duursma, R. A., Blackman, C. J., Lopéz, R., Martin‐StPaul, N. K., Cochard, H., & Medlyn, B. E. (2019). On the minimum leaf conductance: its role in models of plant water use, and ecological and environmental controls. New Phytologist, 221(2), 693-705.