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This protocol describes how to measure gres under constant temperature and relative humidity

Procedure

Materials-Equipments

  • Paraffine wax
  • Scale
  • Scanner
  • ImageJ

Plant material preparation

  • Collect 1 to 1.5 meters long branches
  • Cut the end of each branch underwater
  • Rehydrate plants overnight by immersing them in water
  • Cover the branches with black plastic bags and store them dark conditions

Before starting the measurements

  • Recut a 40 cm long branch (weight should not be larger than 100 grams)
  • Measure the fresh weight of each branch
  • Measure the diameter of the branch at the cut end and at the top of branch
  • Seal the end of the branch with parafilm
  • Check there is water in the reservoir located inside the box
  • Before placing the branches inside the Box,
    • Set the temperature and relative humidity values and wait until these stabilize (You can use this VPD Calculator)
    • Change the TareNow flag (located in the Num Display window) from false to true

During the measurements

Data Analysis

\[E = \frac{slope(n_w;t)}{LA\ +\ BA }\]
\[g_{res}= \frac{E}{VPD}\ \times 101.6\]

Code

import pandas